Seahorse XFe96 Analyze

Agilent Seahorse XFe96 Analyzers measure the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a 96-well plate format.

OCR and ECAR rates are key indicators of mitochondrial respiration and glycolysis as well as ATP production rate. Together these measurements provide a systems-level view of cellular metabolic function in cultured cells and ex vivo samples.

For Research Use Only. Not for use in diagnostic procedures.
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Features

  • 96-well plate format accommodates many conditions in a single run, for flexible assay design, dose-response studies, and screening
  • Reports real-time metabolic rates in minutes, without sample extraction or labelling
  • Four-port injection system with automated mixing function, for detection of live-cell responses to substrates, inhibitors, and other compounds in real time
  • High-sensitivity analysis of as few as 5,000 cells per well using the custom 96-well plate
  • Precision-controlled heating tray, maintains 16–42°C temperatures (12–20°C above ambient conditions), for compatibility with a variety of sample types
  • Quickly determine dependency of cellular energy production on mitochondrial substrates
  • Generate a metabolic phenotype within one hour for fast data turnaround
  • Analyze 3D samples such as spheroids and islets
  • Wave software allows easy assay protocol creation and data analysis on your desktop PC and export to common spreadsheet and graphing programs

Specifications

Amperage
  • 9 A
Analyzer Application
  • Phenotyping Screening
Assay Running Volume
  • 150 – 275 µL/well
Assay Wells
  • 92
Communication
  • TCP/IP; USB; wireless
Controller Description
  • Combination of Windows 10 computer and touchscreen display with full assay design, control, and analysis capability
Controller Touchscreen
  • Yes
Controller Type
  • Cable-connected
Depth
  • 43 cm
Height
  • 61 cm
Injection Ports per Well
  • 4 per well
Memory
  • 16 GB
Microchamber Volume
  • 2.8 µL
Operating Environment Relative Humidity
  • 20 – 80 %
Operating Environment Temperature
  • 4 – 30 °C
Operating Voltage
  • 100 – 240 VAC
Sample Requirements
  • 5000 – 500000 cells/well
Sample Temperature
  • 16 – 42 °C
Software Included
  • Wave Controller
  • Windows 10 Enterprise
  • Wave Desktop unlimited use license
Volume per Port Injection
  • 25 µL
Weight
  • 23 kg
Width
  • 41 cm

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