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Seahorse XF Imaging and Normalization System

The Agilent Seahorse XF Imaging and Normalization system is an integrated solution that acquires brightfield and fluorescence images and calculates cell numbers in each well. It then seamlessly transfers these images and cell counts into Wave software, where they are used to normalize XF data.

This solution provides the evidence needed to filter and better interpret XF data. Complete system integration requires the purchase of Agilent Cytation 1 or Cytation 5 Cell Imaging Multi-mode Readers.


  • Perform XF analysis with an easy-to-use, reliable, validated, and supported cell count based normalization solution
  • Apply cell count numbers directly to your XF data, enabling plate–plate, experiment–experiment, and well–well comparisons that improve XF data interpretation
  • Document the cell culture conditions throughout the XF assay, to quality control assay readiness and assist with finding outliers
  • Associate normalization values with brightfield and fluorescence images in Wave 2.6 software, providing the evidence for more meaningful biological conclusions
  • Enhanced live cell assay reproducibility optimized for XF assays
  • Simplify your normalization workflow with uncomplicated software and single controller operation to communicate data between both devices