Protein Quantitation

The Key to a Successful Bioanalytical Strategy

If you want to succeed in the fastest growing pharmaceutical segment (biotherapeutic proteins), you need to understand the pharmacokinetics and bioequivalence of target drugs.

For that, you’ll need a high-quality protein quantification method.

Recent advances in mass spectrometry have given these powerful tools the sensitivity needed to revolutionize large molecule biologics detection. In fact, protein quantification by mass spectrometry is becoming the method of choice for analyzing biotherapeutics.

The first challenge of protein quantitation from a biological matrix lies with sample preparation and analyte recovery. The most effective technique for purification of biotherapeutic drugs from biological matrices is immunoaffinity purification.

Reproducibility and complete recovery are critical and directly affect the quantification results. You’ll be able to achieve great results with the Agilent AssayMAP Bravo platform, which provides automated and reproducible immunoaffinity purification. The AssayMap Bravo comes with protein A/G and straptavidin cartridges to enable easy and streamlined purification. This versatile platform can also perform denaturation, reduction, alkylation, and digestion—essential steps for quantitation of peptide surrogates.

The second challenge of protein quantitation resides in the need to detect extremely low levels of a wide range of molecular weights, including very large proteins. Designed specifically for large molecules, the Agilent 6545XT AdvanceBio LC/Q-TOF system offers unsurpassed sensitivity and dynamic range for proteins and peptides. It also can perform qualitative confirmation at the same time as quantitation. The Agilent 6495C triple quadrupole LC/MS system is ideal for targeted peptide quantitation.

Agilent provides a complete solution for protein quantitation, including automated sample preparation, separation, detection, and data analysis.

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