Environmental

Analysis of Per and Polyfluoroalkyl Substances in Edible Fish Tissue Using Agilent Captiva EMR–Lipid and LC/MS/MS

This application note presents the development and evaluation of a multicomponent method for the analysis of 25 per- and polyfluoroalkyl substances (PFAS) in fish muscle tissue. The method incorporates a solvent extraction followed by a pass-through lipid removal step using the Agilent Captiva Enhanced Matrix Removal–Lipid (EMR–Lipid) and Quantitative Analysis by LC/MS/MS. The average surrogate recovery for 140 extractions across 25 fish species was 105% with an average relative standard deviation (RSD) of 14%.