Robust and Reproducible Protein Quantification in Plasma using the Evosep One and the Agilent 6495 Triple Quadrupole LC/MS

This application note showcases the reproducibility, robustness, and analytical sensitivity of MRM-based LC/MS analysis of human plasma proteins using the Agilent 6495 triple quadrupole LC/MS with the Evosep One LC system. Results demonstrated superior retention time reproducibility over a 12-day analysis period, and equivalent sensitivity before and after robustness testing, underlining the suitability of the system for high-throughput protein quantification.