In early-stage drug discovery, high-purity compounds are needed for compound screening and lead identification. The Agilent 1290 Infinity II Preparative LC/MSD System, including the Agilent 1290 Infinity II Preparative Open-Bed Sampler/Collector, is ideally suited for purification of crude samples in early-stage drug discovery. The system offers mass-based fraction collection and precise delay calibration, which enables maximum purity and recovery of collected fractions and results in higher confidence in the quality of compounds studied during early stage drug discovery. In this application note, paracetamol is purified from an example crude sample using the 1290 Infinity II Preparative LC/MSD System. Purity and recovery of the collected paracetamol are determined.