Agilent Captiva Premium syringe filters were tested for filtration efficiency by filtering a homogeneous solution of latex beads. The bead particle size selection was based on the syringe filter membrane pore size. Excellent and consistent filtration efficiency (removal of >90% particulates) was demonstrated for the Captiva Premium syringe filters. The filtration impact on an LC column was evaluated on sub-2 µm and superficially porous columns by monitoring the column backpressure with continuous injections of latex beads samples. The results showed that filtration can provide the best protection to LC columns and, thus, lead to considerably longer column lifetime.